Fig. 5. Ability of CepR2 to fold into a soluble form requires GroESL but does not require OHL.
E. coli strain BL21(DE3)(pGR107) containing or lacking pT7-GroESL was cultured in medium containing or lacking 1 μM OHL, lysed, and clarified extracts were size- fractionated by SDS-PAGE and stained using Coomassie Brilliant Blue. Results are representative of three experiments with similar results.