Table 1.
Transcriptional profiles of cells cultured in the presence or absence of 1 μM OHL.a
| OHL Induction Ratio (S.D.) | |||||
|---|---|---|---|---|---|
| Gene | Trial 1 | Trial 2 | Average | cepR2 vs. WT (Malott et al., 2009) | Alternate Name, Comments, References |
| lacZ | 3.51 (0.81) | 3.24 (0.32) | 3.38 | n.a.b | cepI-lacZ reporter |
| bcal0510 | 7.61 (1.58) | 6.41 (0.46) | 7.01 | n.d.c | CepR-regulated (Wei et al., 2011) |
| bcal0831 | 1.66 (0.20) | 2.88 (0.81) | 2.27 | n.d. | |
| bcal0833 | 0.87 (0.15) | 1.29 (0.28) | 1.08 | n.d. | phbB |
| bcal2118 | 1.55 (0.24) | 3.56 (0.93) | 2.55 | n.d. | |
| bcal3178 | 1.49 (0.41) | 3.09 (0.54) | 2.29 | n.d. | |
| bcam0030 | 2.63 (1.41) | 5.36 (2.27) | 3.99 | n.d. | |
| bcam0031 | 2.57 (1.21) | 4.48 (3.18) | 3.52 | n.d. | |
| bcam0184 | 1.34 (0.28) | 4.06 (3.23) | 2.70 | n.d. | Lectin |
| bcam0185 | 1.43 (0.45) | 11.8 (1.96) | 6.59 | n.d. | Lectin |
| bcam0186 | 3.12 (0.40) | 1.81 (0.52) | 2.46 | 2.9 (0.8) | bclA (lectin) |
| bcam0187 | 0.99 (0.22) | 3.24 (0.32) | 2.12 | n.d. | |
| bcam0188 | 2.45 (0.99) | 2.62 (0.69) | 2.53 | 21.1 (3.4) | cepR2 |
| bcam0189 | 1.03 (0.26) | 2.15 (0.46) | 1.59 | 188 (46) | cepS |
| bcam0190 | 2.00 (0.81) | 3.25 (0.54) | 2.62 | 11.6 (0.9) | Aminotransferase Class III |
| bcam0191 | 1.96 (0.37) | 4.11 (1.24) | 3.04 | 9.5 (3.0) | Non-ribosomal peptide synthase |
| bcam0192 | 4.92 (1.24) | 4.63 (2.79) | 4.78 | 113 (27) | Conserved hypothetical |
| bcam0193 | 3.08 (1.28) | 6.15 (1.73) | 4.62 | 171 (74) | Conserved hypothetical |
| bcam0194 | 7.46 (1.69) | 5.18 (1.70) | 6.32 | 151 (48) | Conserved hypothetical |
| bcam0195 | 3.41 (1.76) | 6.60 (1.33) | 5.00 | 58.3 (62) | Non-ribosomal peptide synthase |
| bcam0196 | 7.08 (3.02) | 3.50 (3.19) | 5.29 | 80.5 (31) | Conserved hypothetical |
| bcam0393 | 2.45 (0.89) | 3.34 (1.43) | 2.90 | n.d. | |
| bcam0634 | 3.59 (0.08) | 2.64 (0.15) | 3.12 | n.d. | |
| bcam1413a | 3.58 (1.60) | 3.50 (3.19) | 3.54 | n.d. | aidC (Wei et al., 2011) |
| bcam1742 | 1.86 (0.65) | 2.60 (1.10) | 2.23 | n.d. | |
| bcam1869 | 1.86 (0.65) | 3.26 (1.23) | 2.56 | n.d. | CepR-regulated (Wei et al., 2011) |
| bcam2307 | 2.96 (0.60) | 7.09 (3.23) | 5.02 | −1.7 ± (0.7) | zmpB |
| bcam2308 | 2.00 (0.57) | 2.31 (0.85) | 2.15 | n.d. | |
| bcas0153 | 2.34 (0.42) | 1.78 (0.21) | 2.06 | n.d. | |
| bcas0292 | 14.9 (11.8) | 39.4 (41.2) | 27.2 | n.d. | aidB, CepR-regulated (Wei et al., 2011) |
| bcas0293 | 293 (299) | 198 (156) | 245 | 1.7 ± (0.2) | aidA, CepR-regulated (Wei et al., 2011) |
| bcas0409 | 2.31 (0.60) | 2.64 (0.67) | 2.47 | 1.6 ± (0.3) | zmpA |
| All Genes | 1.00 (1.26) | 1.00 (1.37) | 1.00 | ||
a
Strain CLW101 contains a chromosomal PcepI-lacZ fusion (Weingart et al., 2005). In Trial 1 the culture lacking OHL expressed 2.7 Miller units of β-galactosidase, while the culture containing OHL expressed 290 units. In Trial 2, the culture lacking OHL expressed 1.5 units, while the culture containing OHL expressed 195 units.
b
Not applicable
c
Not determined