Table 3.
Regulated expression of the bcam0191 and bcam0192 promoters in E. colia.
| Fusionb | Plasmids expressing B. cenocepacia genes | OHL (uM) | β-Galactosidase Activity | Normalized Valuec |
|---|---|---|---|---|
| bcam0191 | none | 0 | 121 ± 11 | (1) |
| None | 1 | 137 ± 15 | 1.13 | |
| pGR192 (cepR2) | 0 | 29 ± 8 | 0.24 | |
| pGR192 (cepR2) | 1 | 35 ± 8 | 0.29 | |
| pGR276 (cepS) | 0 | 310 ± 21 | 2.6 | |
| pGR276 (cepS) | 1 | 305 ± 17 | 2.5 | |
| pGR192 (cepR2), pGR276 (cepS) | 0 | 35 ± 8 | 0.29 | |
| pGR192 (cepR2), pGR276 (cepS) | 1 | 307 ± 30 | 2.5 | |
| bcam0192 | None | 0 | 101 ± 11 | (1) |
| None | 1 | 93 | 0.92 | |
| pGR192 (cepR2) | 0 | 23 ± 4 | 0.23 | |
| pGR192 (cepR2) | 1 | 32 ± 3 | 0.32 | |
| pGR276 (cepS) | 0 | 300 ± 11 | 3.0 | |
| pGR276 (cepS) | 1 | 344 ± 23 | 3.4 | |
| pGR192 (cepR2), pGR276 (cepS) | 0 | 32 ± 3 | 0.32 | |
| pGR192 (cepR2), pGR276 (cepS) | 1 | 293 ± 30 | 2.9 |
a
All strains were derived from MC4100. Strains were cultured at 37°C in LB supplemented with 0.5 mM IPTG, appropriate antibiotics, and containing or lacking OHL as indicated to an OD600 of 0.4, and assayed for β-galactosidase activity. Data were obtained from a single representative experiment using three independent isolates of each strain, each assayed once. Mean value and standard deviations are indicated.
b
A bcam0191-lacZ transcriptional fusion was provided by pGR130, while a bcam0192-lacZ fusion was provided using pGR136.
c
β-galactosidase activity is normalized to that of the wild type strain carrying the indicated plasmid and cultured in the absence of OHL.