Table 4.
Regulation of resected bcam0191 promoters by CepR2, CepS, and OHLa.
| Plasmid | Fragment | Genotype | OHL (uM) | β-Galacto- sidase | Normalized Valueb |
|---|---|---|---|---|---|
| pGR130 | −395 – +98 | WT | 0 | 35 ± 8 | (1) |
| WT | 1 | 377 ± 30 | 10.8 | ||
| GR141 (cepR2-) | 0 | 381 ± 30 | 10.9 | ||
| GR141 (cepR2-) | 1 | 365 ± 17 | 10.4 | ||
| GR145 (cepS-) | 0 | 7 ± 1 | 0.2 | ||
| GR145 (cepS-) | 1 | 2 ± 0 | 0.01 | ||
| pGR132 | −207 – +98 | WT | 0 | 94 ± 27 | (1) |
| WT | 1 | 361 ± 34 | 3.8 | ||
| GR141 (cepR2-) | 0 | 327 ± 28 | 3.5 | ||
| GR141 (cepR2-) | 1 | 349 ± 31 | 3.7 | ||
| GR145 (cepS-) | 0 | 9 ± 1 | 0.1 | ||
| GR145 (cepS-) | 1 | 7 ± 1 | 0.07 | ||
| pGR195 | −184 – +98 | WT | 0 | 74 ± 13 | (1) |
| WT | 1 | 361 ± 35 | 4.9 | ||
| GR141 (cepR2-) | 0 | 326 ± 28 | 4.4 | ||
| GR141 (cepR2-) | 1 | 389 ± 31 | 5.3 | ||
| GR145 (cepS-) | 0 | n.d. | n.d. | ||
| GR145 (cepS-) | 1 | n.d. | n.d. | ||
| pGR133 | −128 – +98 | WT | 0 | 339 ± 9 | (1) |
| WT | 1 | 361 ± 20 | 1.1 | ||
| GR141 (cepR2-) | 0 | 339 ± 29 | 1.0 | ||
| GR141 (cepR2-) | 1 | 360 ± 11 | 1.1 | ||
| GR145 (cepS-) | 0 | 1 ± 1 | 0.003 | ||
| GR145 (cepS-) | 1 | 1 ± 1 | 0.002 | ||
| pGR134 | −90 – +98 | WT | 0 | 10 ± 2 | (1) |
| WT | 1 | 6 ± 4 | 1.0 | ||
| GR141 (cepR2-) | 0 | 9 ± 4 | 1.0 | ||
| GR141 (cepR2-) | 1 | 10 ± 3 | 1.0 | ||
| GR145 (cepS-) | 0 | 2 ± 1 | 0.2 | ||
| GR145 (cepS-) | 1 | 2 ± 1 | 0.2 | ||
| pGR236 | −395 – 27 | WT | 0 | 45 ± 6 | (1) |
| WT | 1 | 248 ± 26 | 5.5 | ||
| GR141 (cepR2-) | 0 | 248 ± 19 | 5.5 | ||
| GR141 (cepR2-) | 1 | 226 ± 20 | 5.0 | ||
| GR145 (cepS-) | 0 | 3.3 ± 1 | 0.07 | ||
| GR145 (cepS-) | 1 | 2.5 ± 1 | 0.06 |
a
Derivatives of strain K56-I2 containing the indicated cepR2 or cepS mutations and the indicated plasmids were cultured at 37° C in LB supplemented with 0.5 mM IPTG and 300 μg ml-1 tetracycline, in the presence or absence of 1 μM OHL to an optical density of approximately 0.4 and assayed for β-galactosidase activity. Data were obtained from a single representative experiment using three independent isolates of each strain, each assayed once. Mean value and standard deviations are indicated.
b
β-galactosidase activity is normalized to that of the wild type strain carrying the indicated plasmid and cultured in the absence of OHL.