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. 2013 Jan;20(1):1–8. doi: 10.1128/CVI.00280-12

Fig 1.

Fig 1

Comparison of immune responses in A/J mice induced by Ad5 vectors encode codon-optimized and non-codon-optimized PA or LF genes. (A to C) Anti-PA IgG ELISA titer (A), anti-LF IgG ELISA titer (B), and anti-Letx neutralizing antibody titer (C) in mouse serum were compared 4 weeks after a single i.n. immunization with AdPA83 (PA), AdPA83hu (PAhu), AdtPA83hu (tPAhu), AdmLF7 (mLF7), and AdtLFnhu (tLFnhu) at a dose of 107 IFU per vector. All mice immunized with Adtetc control vectors and all preimmune sera had anti-PA or anti-LF IgG ELISA titers of ≤100 and had anti-Letx neutralizing antibody titers of <8. (D) Survival of vaccinated A/J mice against a 50× LD50 B. anthracis Sterne spore challenge. The results of anti-PA and anti-LF IgG ELISAs and anti-Letx neutralizing assays are plotted as the log10 titers. Bars are geometric mean of the anti-PA and anti-LF ELISA titers or the anti-Letx neutralizing antibody titers.