Table 1.
Name | Sequence | Linker | Modification | Mol wt |
|||
---|---|---|---|---|---|---|---|
Free peptide |
CHEMS conjugated |
||||||
Experimental | Observed | Experimental | Observed | ||||
C-MPER | 671–683 | AA | None | 2,537.1 | 2,539.6 | ||
C-MPERext | 667–683 | AA | None | 2,526.0 | 2,550.1 (Na+) | 2,994.6 | 2,993.9 |
C-MPERext(S-PO3) | 667–683 | AA | S-PO3 | 2,605.0 | 2,609.3 | 3,077.6 | 3,075.2 |
C-MPER(Y-PO3) | 671–683 | AA | Y-PO3 | 2,147.4 | 2,151.0 | 2,615.1 | 2,617.0 |
C-MPER(Y-NO2) | 671–683 | AA | Y-NO2 | 2,113.4 | 2,106.7 | 2,585.2 | 2,583.2 |
N-MPER | 656–671 | GG | None | 2,271.5 | 2,273.5 | 2,698.3 | 2,718.2 (Na+) |
N-MPER(D) | 656–677 | GG | S668D | 2,299.5 | 2,307.2 | ||
N-MPER(E) | 656–677 | GG | S668E | 2,313.6 | 2,336.8 (Na+) | ||
N-MPER(S-PO3) | 656–671 | GG | S-PO3 | 2,308.5 | 2,313.6 | 2,776.2 | 2,776.7 |
N-MPERext(T-PO3) | 656–677 | GG | T-PO3 | 3,085.4 | 3,087.3 | 3,554.0 | 3,556.3 |