Fig 2.

Antisera from FIS-immunized mice react with purified spore proteins. (A) Twenty-nine spore proteins were purified, blotted to a nitrocellulose membrane in triplicate, and probed with the day 28 prechallenge antisera from the different immunization groups. The format for each dot blot is shown at the top left. (B) Normalized signal intensities for the 569-UM20 FIS, dcG9241 FIS, or dcG9241 ΔbclA FIS antisera that reacted with proteins from the spore protein panel. The error bars represent one standard error of the mean.