Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| Top10 | High-competency cloning strain | Life Technologies |
| INV110 | dam dcm mutant strain used to produce unmethylated plasmid DNA | Life Technologies |
| B. anthracis | ||
| Ames | pXO1+/pXO2+ | 30 |
| Ames ΔbclA | pXO1+/pXO2+ ΔbclA::kan | 39 |
| Sterne 34F2 | pXO1+/pXO2− | NMRCa |
| B. cereus | ||
| 569-UM20 | Plasmidless, nonencapsulated, anthranilic acid-negative strain derived from B. cereus 569 | 40 |
| dcG9241 | pBCXO1−/pBC210−/pBClin29+ | 18 |
| dcG9241 ΔbclA | pBCXO1−/pBC210−/pBClin29+ ΔbclA::kan | This work |
| Plasmids | ||
| pGEM-T | TA-based E. coli cloning vector; Ampr | Promega |
| pUTE583 | Dual E. coli and Bacillus vector; Cmr in E. coli, Emr in Bacillus | 41 |
| pUTE618 | Source of Ωkan cassette for gene replacement; Cmr Spr | 41 |
| pBclA-U | 1-kb region upstream from bclA in pGEM-T; Ampr | This work |
| pBclA-D | 1-kb region downstream from bclA in pGEM-T; Ampr | This work |
| pBclA-UD | 1-kb regions upstream and downstream of bclA in pGEM-T; Ampr | This work |
| pBclA-UΩD | Ωkan cassette between 1-kb regions upstream and downstream of bclA in pGEM-T; Ampr | This work |
| p583BclA-UΩD | Ωkan cassette between 1-kb region upstream and downstream of bclA in pUTE583; Cmr in E. coli, Emr in Bacillus | This work |
a
NMRC, Naval Medical Research Center.