Fig 3.
The rho1G15V and rho1Q64L point mutants as well as both rho10Δ and rho10Δ rho11Δ strains are temperature sensitive, and this sensitivity is remediated by osmotic stabilization only for strains containing a deletion of RHO10. Cultures of strains were grown overnight in YPD medium at 30°C then diluted to an OD650 of 1.0. Five microliters of 10-fold serial dilutions were plated onto YPD plates with or without 1 M sorbitol (Sorb) and grown at indicated temperatures for 5 days. Strain names or genotypes are indicated to the left of each panel.