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A strain with a deletion of RHO10 but not both RHO10 and RHO11 is sensitive to nitrosative stress. Cultures of strains were grown overnight in YPD medium at 30°C and then diluted to an OD650 of 1.0. Five microliters of 10-fold serial dilutions were plated onto freshly made YNB, pH 4.0, plates containing 1 mM NaNO2 and grown for 5 days at 30°C.
