Table 1.
Primers used in this study
| Primer no. | Primer designationb | Primer sequence |
|---|---|---|
| Primers for amplification of MID1 cDNA | ||
| 1 | MID1-cDNA-F | ATGCCAGCGAGAGAGGTGTA |
| 2 | MID1-cDNA-R | CTATCCGTTACACCATCTAT |
| Primers for construction of MID1 point mutations | ||
| 3 | MID1-F-point mutations | ATGCCAGCGAGAGAGGTGTATTTCAAA |
| 4 | MID1-R-R619A | TTCCGTTACACCATGCATTTCCCCAGCGATCTTGTGCGGT |
| 5 | MID1-R-C621A | TTCCGTTAGCCCATCTATTTCCCCAGCGATCTTGTGCGGT |
| 6 | MID1-R-S605A | TTCCGTTACACCATCTATTTCCCCAGCGATCTTGTGCGGTGACCCCTTGCTCTGCGGCCCGTC |
| Primers for construction of MID1 truncated mutantsa | ||
| 7 | MID1-R-CA-124 | GAAGCGTCTCTGATCGGTTTTGAACCGA |
| 8 | MID1-R-CB-91 | GTTTATGGCGCATATAGAGTTGTCCAAA |
| 9 | MID1-R-CC-24 | GCCGGCACAACCAGTCTCTGTAAGCAGA |
a
The forward primer used to make the MID1 truncated mutants was primer 1.
b
R indicates a reverse primer (3′ to 5′ direction), and F indicates a forward primer (5′ to 3′ direction).