Sexual development of B. dermatitidis. After coculture of B. dermatitidis isolates ATCC 18188 (+) and ATCC 18187 (−) on SEA medium for 10 weeks, cleistothecia were scraped off the agar, disrupted in water, and fixed in formaldehyde prior to microscopic examination. (A) 20× light microscopy shows multiple disrupted cleistothecia, with highly spiraled hyphae. (B) 40× image showing thickened, coiled hyphae and specialized vegetative cells attached to the spiral hyphae. (C) 60× image shows multiple ascospores released from asci. The ascospores are characterized by a light halo around the cell.