Table 3.
Evidence of genetic recombination of the progeny of B. dermatitidis ATCC18187 and ATCC18188a
| Isolate | PCR-RFLP 1 | PCR-RFLP 2 | Mating type | RAPD 1 | RAPD 2 | Recombination |
|---|---|---|---|---|---|---|
| 18188 (P1) | 1 | 1 | 1 | 1 | 1 | Not applicable |
| 18187 (P2) | 2 | 2 | 2 | 2 | 2 | Not applicable |
| M1-1 | 1 | 1 | 1 | 1 | 1 | Not detected |
| M1-2 | 1 | 1 | 1 | 2 | 2 | Yes |
| M1-3 | 1 | 1 | 1 | 1 | 1 | Not detected |
| M1-4 | 1 | 2 | 2 | 2 | 2 | Yes |
| M1-5 | 1 | 1 | 2 | 1 | 1 | Yes |
| M1-6 | 1 | 1 | 1 | 1 | 1 | Not detected |
| M1-7 | 2 | 1 | 1 | 1 | 1 | Yes |
| M1-8 | 1 | 1 | 1 | 1 | 1 | Not detected |
| M1-9 | 1 | 1 | 1 | 1 | 1 | Not detected |
| M1-10 | 1 | 1 | 1 | 1 | 1 | Not detected |
| M2-1 | 2 | 2 | 1 | 1 | 1 | Yes |
| M2-2 | 2 | 2 | 1 | 1 | 1 | Yes |
| M2-3 | 1 | 1 | 2 | 2 | 2 | Yes |
| M2-4 | 2 | 1 | 1 | 1 | 2 | Yes |
| M2-6 | 1 | 1 | 1 | 2 | 2 | Yes |
| M2-7 | 2 | 1 | 1 | 1 | 1 | Yes |
| M2-11 | 1 | 1 | 2 | 2 | 2 | Yes |
| M2-16 | 2 | 2 | 1 | 1 | 1 | Yes |
| M2-17 | 1 | 2 | 2 | 1 | 1 | Yes |
| M2-18 | 2 | 1 | 2 | 1 | 1 | Yes |
| M2-19 | 2 | 2 | 2 | 1 | 2 | Yes |
| M2-20 | 1 | 2 | 1 | 1 | 1 | Yes |
a
The mating types were determined by PCR assay (see Fig. 3). Amplification of an alpha-box gene is determined as mating type 1 (MAT1-1), and amplification of an HMG gene is designated as mating type 2 (MAT1-2).