Fig 1.

PKH2-02 affects high-temperature growth and is required for cellular integrity. (A) A 10-fold dilution series of the indicated strains was spotted on YPD plates or YPD containing SDS (0.6%) and incubated at 30°C or 37°C for 3 days. (B) Cells from an overnight culture were incubated with trypan blue (0.4%) at a 1:1 dilution for 15 min. One hundred cells were counted per field and scored. There was statistical significance between the WT and the pkh2-02Δ mutant (P = 0.0005) but not between the WT and the pkh2-02::PKH2 strain (chi-square test, P > 0.05). (D) The indicated strains were streaked on plates containing YPD or YPD supplemented with 1 M sorbitol and incubated at 37°C for 3 days.