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PKH2-02 is required for oxidative and nitrosative stress tolerance. (A) A 10-fold dilution series of the indicated strains was spotted on YNB (pH 4.0) supplemented with H2O2 (1.5 mM), NaNO2 (1.5 mM), or diamide (1 mM). (B and C) Cell lysates from log-phase cells exposed to either NaNO2 (1 mM) or H2O2 (1 mM or 10 mM) were analyzed for Mpk1 phosphorylation utilizing phospho-p44/42 MAPK. Anti-PSTAIRE and anti-Tsa1 are loading controls.
