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PKH2-02 is required for virulence in the G. mellonella model. G. mellonella larvae were injected in the terminal pseudopod with WT, pkh2-02Δ, and pkh2-02Δ::PKH2-02 strains at a concentration of 2 × 105 CFU/larva and incubated at 30°C. Survival was scored every 24 h. The survival difference between the WT and pkh2-02Δ strains was statistically significant (P < 0.05 by the Kaplan-Meier test).
