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. 2012 Dec 18;2012:819632. doi: 10.1155/2012/819632

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Copyright © 2012 Hsue-Yin Hsu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells. (a) Cells were collected at the indicated time points after treatment with 1.25 mg/mL MCME. Time-dependent effects of MCME on the protein expression level of PI3K, Akt, Wnt-2, GSK-3β, phosphorylated GSK-3β, β-catenin, and vimentin in CL1-0 and CL1-5 cells were evaluated by western blot. (b) The protein expression levels were normalized to β-actin and expressed as the fold-change to the respective control. Each value represented by the mean ± SEM. is quantified by representative blots from 3 independent experiments. * indicates a significant difference of P < 0.01 as compared with control groups, whereas ^# indicates P < 0.05 between two groups.