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Determination of specific anti-ROP13 antibody titers in the sera of Kunming mice immunized with 100 μl of PBS; 100 μg of pVAX I, pVAX I/IL-18, or pVAX-ROP13 alone; or 50 μg of pVAX I/IL-18 and pVAX-ROP13 at weeks 0, 2, 4, and 6. Sera were collected 1 day prior to each immunization and tested by ELISA using STAg. The results are shown as means of three independent experiments. Statistically significant differences (P < 0.05) are indicated by asterisks.
