Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Oligonucleotide primers used for construction of the MIC1-MAG1-SAG1 chimeric antigen
| T. gondii gene(s) | Primer name | Primer sequence | Underlined sequence | Template for amplification |
|---|---|---|---|---|
| mic1/mag1 | M1 (forward) | 5′-GTGCCAGATCTAGCGTCGCATTCTCATTCG-3′ | BglII | pUET1/MIC1-MAG1 |
| M2 (reverse) | 5′-GGCAACAAGAGGGGGATCACCAGATCCCTGAACCC-3′ | Fragment of sag1 | ||
| sag1 | S1 (forward) | 5′-GGGTTCAGGGATCTGGTGATCCCCCTCTTGTTGCC-3′ | Fragment of mag1 | pUET1/SAG1 |
| S2 (reverse) | 5′-GCGGCCGCAAGCTTAAGAGTGCTGTCTGCAC-3′ | HindIII |
