Table 3.
Immunoreactivities of the two chimeric antigens (MIC1-MAG1 and MIC1-MAG1-SAG1) or the mixture of recombinant proteins (M)a with IgG antibodies in selected sera from group III (subgroup IIIC)
| Sample no. | IgG level (IU/ml)b | IgG ELISA (OD492)c |
||
|---|---|---|---|---|
| MIC1-MAG1 | M | MIC1-MAG1-SAG1 | ||
| 1 | 18 | 0.397 | 0.510 | 0.767 |
| 2 | 31 | 0.391 | 0.374 | 0.775 |
| 3 | 10 | 0.297 | 0.349 | 0.468 |
| 4 | 28 | 0.409 | 0.561 | 0.866 |
| 5 | 12 | 0.272 | 0.420 | 0.533 |
| 6 | 9 | 0.404 | 0.320 | 0.662 |
| 7 | 8 | 0.408 | 0.345 | 0.508 |
| 8 | 26 | 0.420 | 0.622 | 0.820 |
| 9 | 50 | 0.282 | 0.525 | 1.035 |
| 10 | 44 | 0.263 | 0.547 | 0.871 |
a
M antigen is a mixture of rMIC1ex2 plus rMAG1 plus rSAG1.
b
Determined using a commercial test (Vidas; bioMérieux). Positive results were defined as values above or equal to 8 IU/ml.
c
Positive results (indicated in bold type) were defined as ODs above cutoff values (0.428 for MIC1-MAG1, 0.428 for M, and 0.434 for MIC1-MAG1-SAG1).