Fig 1.

(A) Colonies of Leptospira interrogans serovar Pyrogenes strain NR-20157 on LVW agar. Plates were drop inoculated with 10 μl of bacteria at an approximate concentration of 10⁵, 10⁴, 10³, or 10² CFU/ml (A, B, C, and D, respectively) and incubated at 30°C in 5% CO₂ for 2 days followed by 30°C in air for a total of 28 days. (B) Penicillin G Etest on LVW agar for Leptospira interrogans serovar Autumnalis strain NR-20161. The plate was prepared by spreading 300 μl of 10⁸ CFU/ml and incubated at 30°C in 5% CO₂ for 2 days followed by 30°C in air for a total of 7 days.