Table 2.
Reproducibility of Etest MIC for Leptospira speciesa
| Antimicrobial | Strain | MIC determined by Etest |
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.004 | 0.006 | 0.008 | 0.012 | 0.016 | 0.023 | 0.032 | 0.047 | 0.064 | 0.094 | 0.125 | 0.19 | 0.25 | 0.38 | 0.5 | 0.75 | 1 | ||
| Penicillin G | NR-20161 | 1 | 2 | 1 | ||||||||||||||
| NR-20151 | 2 | 2 | ||||||||||||||||
| NR-20327 | 2 | 2 | ||||||||||||||||
| Doxycycline | NR-20161 | 2 | 2 | |||||||||||||||
| NR-20151 | 1 | 3 | ||||||||||||||||
| NR-20327 | 2 | 2 | ||||||||||||||||
| Cefotaxime | NR-20161 | 1 | 1 | 2 | ||||||||||||||
| NR-20151 | 2 | 2 | ||||||||||||||||
| NR-20327 | 4 | |||||||||||||||||
| Ceftriaxone | NR-20161 | 1 | 3 | |||||||||||||||
| NR-20151 | 3 | 1 | ||||||||||||||||
| NR-20327 | 4 | |||||||||||||||||
| Chloramphenicol | NR-20161 | 1 | 3 | |||||||||||||||
| NR-20151 | 2 | 2 | ||||||||||||||||
| NR-20327 | 1 | 3 | ||||||||||||||||
a
Values are in μg/ml except for penicillin G values, which are in units/ml. Four technicians independently performed the assay for three isolates (strains NR-20161, NR-20151, and NR-20327) and five antimicrobial drugs. Each technician prepared their own Leptospira inoculum, used a different batch of LVW agar, and was blinded to the results of the other technicians.