Table 3.
Interday coefficients of variation and accuracy of determination of various antifungals or metabolite in plasmaa
| Antifungal or metabolite | Sample | Concn (mg/liter) |
% Bias | % Imprecision (CV) | |
|---|---|---|---|---|---|
| Spiked | Found (mean ± SD) | ||||
| Voriconazole | LLOQ | 0.05 | 0.052 ± 0.0028 | 3.2 | 5.4 |
| QC1 | 0.20 | 0.204 ± 0.069 | 1.8 | 3.4 | |
| QC2 | 2.00 | 2.02 ± 0.109 | 1.1 | 5.4 | |
| QC3 | 10.0 | 10.0 ± 0.239 | 0.4 | 2.4 | |
| Posaconazole | LLOQ | 0.05 | 0.051 ± 0.0026 | 2.2 | 5.0 |
| QC1 | 0.20 | 0.201 ± 0.0076 | 0.3 | 3.8 | |
| QC2 | 2.00 | 2.04 ± 0.102 | 1.9 | 5.0 | |
| QC3 | 10.0 | 10.4 ± 0.227 | 4.1 | 2.2 | |
| Itraconazole | LLOQ | 0.05 | 0.052 ± 0.0023 | 3.0 | 4.5 |
| QC1 | 0.20 | 0.202 ± 0.0068 | 0.9 | 3.4 | |
| QC2 | 2.00 | 2.02 ± 0.074 | 1.2 | 3.7 | |
| QC3 | 10.0 | 10.1 ± 0.223 | 1.3 | 2.2 | |
| Hydroxyitraconazole | LLOQ | 0.05 | 0.051 ± 0.0041 | 2.2 | 8.1 |
| QC1 | 0.20 | 0.196 ± 0.0082 | −2.0 | 4.2 | |
| QC2 | 2.00 | 1.95 ± 0.051 | −2.7 | 2.6 | |
| QC3 | 10.0 | 10.0 ± 0.268 | 0.5 | 2.7 | |
a
Interday coefficients of variation (% CV) and accuracy (% bias) of the determination of voriconazole, posaconazole, itraconazole, and hydroxyitraconazole in plasma (n = 6).