Fig 2.

Analysis of cell viability in P. aeruginosa biofilms following alginate lyase treatments. (A) Mucoid FRD1 biofilms were treated for 12 h with either 500 μg/ml tobramycin (Tob), 1,000 μg/ml alginate lyase, or a combination thereof. Subsequently, cell viability was quantified with alamarBlue, and the results were normalized to untreated biofilms. (B) Nonmucoid SMC406 biofilms were treated in the same manner as for panel A, except that tobramycin concentrations were lowered to 100 μg/ml. The error bars represent standard deviations from four replicates. Statistically significant (α = 0.05) differences relative to untreated biofilms are marked with asterisks.