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Pseudo Michaelis-Menten kinetic parameters with BSWA and bacterial (FRD1) alginate substrates
| Enzyme | Substrate | Vmax [ΔA235 (min · mg)−1] | Km (μg/ml) | Vmax/Km |
|---|---|---|---|---|
| AlgL | BSWA | 26 ± 1 | 70 ± 10 | 0.36 ± 0.05 |
| AlgL-His | BSWA | 35 ± 2 | 40 ± 10 | 0.8 ± 0.2 |
| FRD1 | 127 ± 6 | 840 ± 70 | 0.15 ± 0.01 | |
| Al-III | BSWA | 290 ± 20 | 39 ± 9 | 8 ± 2 |
| Al-III–His | BSWA | 337 ± 9 | 100 ± 10 | 3.4 ± 0.4 |
| FRD1 | 310 ± 10 | 420 ± 40 | 0.74 ± 0.08 |
