Fig 4.

Optimization of lipid catalyst. Comparison of the Hz (β-hematin) catalytic efficiency of PS (l-α-phosphatidyl-l-serine), SG (1-monostearin, sMAG), DO (1,2-diolyl-rac-glycerol [18:1, cis-9]), DT (1,2-dioctanoyl-sn-glycerol-3-PC), PG (1-monopalmitin, pMAG), DG (1,2-dipalmitoyl-sn-glycerol-3-PC), PC (l-α-phosphatidylcholine), and BD (blend, meaning an equimolar mixture of PC, pMAG, and sMAG resulting in 1 mM/well final concentration). FPIX (100 μM) was incubated in 1 M propionate buffer (at the different pH values) at 37°C in the presence of 1 mM the corresponding lipid. The amount of Hz formed in the corresponding lipid wells (average of triplicate wells) was quantified relative to that for a lipid-free control after a 16-h incubation period. The 100% Hz yield was calculated via equation 1, with the sample absorbance being that of 100 μM FPIX.