Fig 3.

Plasma concentrations of tigecycline in rats treated with a single dose (filled symbols) or 5 consecutive doses (open symbols) of 6.25 mg/kg (squares) or 12.5 mg/kg (circles). Blood samples of rats were taken at 0, 15, and 30 min and 1, 2, 4, 8, 12, and 24 h after administration of tigecycline. The concentration at each time point was determined in triplicate. Concentrations are expressed as means ± standard deviations (SDs). The limit of quantification of tigecycline in rat plasma samples was 0.0114 μg/ml. The response from calibration standards was linear from 0.0114 to 15.0 μg/ml, and the coefficient of correlation for all measured sequences was at least 0.995. The intraday precision and analytical recovery of the tigecycline assay during study sample analysis ranged from 2.9 to 7.7% and from 100.8 to 104.5%, respectively. Concentration-time curves were analyzed using WinNonlin employing a two-compartment model.