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Plot of 1/[E] versus time for the interaction between 0.1 μM R6 PBP2x and 0.1 μM ceftaroline. Incubation was performed at 30°C in 10 mM sodium phosphate buffer, pH 7.0, in the presence of 1 mM S2d and 0.5 mM 4,4′-dithiodipyridine. The hydrolysis of S2d was monitored at 324 nm. The rate of spontaneous hydrolysis of S2d was subtracted.
