Table 2.
Primers used in the study
| Primer | Nucleotide sequence (5′–3′) |
|---|---|
| INA-axyY-F | CGGTACCCGGGGATCCAGGGCAGCTTCATGGCCATa |
| INA-axyY-R | CGACTCTAGAGGATCGGGAAGCCGTTGTAGCGGTTa |
| V-INA-axyY-F | CGCCTGACCACGCGCTATA |
| M14Fb | CCAGGGTTTTCCCAGTCACGA |
| M14Rb | GCGGATAACAATTTCACACAGGA |
| V-INA-axyY-R | CGGACAGCCGCTCTTCGTA |
| oprZ-F | CACGCTCAAGCTGACCCA |
| oprZ-R | CCTCCAGCATCTCCAGGT |
a
Underlined sequences comprise 15 bases of homology with the ends of the linearized vector pUC19.
b
M14F and M14R are primers designed in suicide plasmids.