Table 2.
Highest probable matches for the 13 variable interspersed DNA sequences in the clustered regularly interspaced short palindromic repeat (CRISPR) region in ST779/t878 MRSA isolatesa generated using the online CRISPRfinder software tool
| CRISPR repeat no. | BLASTn resultb | GenBank accession no. | % DNA sequence similarity | % query coverage |
|---|---|---|---|---|
| 1 | Phenylobacterium zucineum HLK1 plasmid | CP000748 | 100 | 60 |
| 2 | Geobacillus thermoleovorans | CP003125 | 100 | 86 |
| 3 | Megamonas hypermegale | FP929048 | 100 | 66 |
| 4 | Bacteroides xylanisolvens XB1A | FP929033 | 100 | 100 |
| 5 | Staphylococcus haemolyticus | AP006716 | 100 | 93 |
| 6 | S. haemolyticus | AP006716 | 100 | 93 |
| 7 | Shewanella piezotolerans | CP000472 | 100 | 80 |
| 8 | S. haemolyticus | AP006716 | 93 | 100 |
| 9 | Escherichia blattae | CP001560 | 100 | 56 |
| 10 | Methylophaga sp. | CP003380 | 100 | 70 |
| 11 | S. epidermidis plasmid | GQ900454 | 100 | 86 |
| 12 | S. haemolyticus | AP006716 | 100 | 93 |
The CRISPR regions of six isolates were sequenced (isolates M06/0171, M09/0295, M08/0422, M11/0208, M09/0302, and E4449).
The BLASTn algorithm was used to search for similar sequences in GenBank compared to each of the variable interspersed repeats in the CRISPR region in the novel SCCCRISPR element.