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Polymicrobial biofilm formation leads to synergistic increases in biomass. Monomicrobial and polymicrobial C. albicans (CA) and/or S. aureus (SA) biofilms were grown on polystyrene plates for 24 h. Biomass was quantified by the crystal violet method. The biomass of the polymicrobial biofilm was significantly higher than the additive biomasses of the monomicrobial biofilms. *, P < 0.05, using Student's t test.
