Fig 2.

Optimized dosing and time course of activity of ethanol against monomicrobial and polymicrobial biofilms grown on polystyrene. Various concentrations of EtOH were added to preformed monomicrobial (A, B) and polymicrobial (C) biofilms of C. albicans and/or S. aureus for various incubation times. (D) Initial biofilm inocula were treated with various concentrations of EtOH for 24 h to inhibit growth. Metabolic activity was assessed using the XTT assay, and data are expressed as the percentage of the value for the untreated controls. Error bars represent SDs. * and # (entire group), P < 0.05, using one-way ANOVA and Dunnett's posttest.