Table 1.
Regrowth of biofilms after treatment with ethanola
| EtOH concn (%) | Monomicrobial |
Polymicrobial |
||||||
|---|---|---|---|---|---|---|---|---|
|
C. albicans |
S. aureus |
C. albicans |
S. aureus |
|||||
| OD600 | No. of CFU/ml | OD600 | No. of CFU/ml | OD600 | No. of CFU/ml | OD600 | No. of CFU/ml | |
| 0 | 1.182 | > 108 | 0.266 | > 108 | 1.187 | > 108 | 0.204 | > 108 |
| 10 | 0.961 | > 108 | 0.355 | > 108 | 1.006 | > 108 | 0.169 | 2.6 × 107 |
| 30 | 0.000 | 0 | 0.005 | 1.2 × 106 | 0.000 | 0 | 0.010 | 3.1 × 106 |
| 50 | 0.000 | 0 | 0.000 | 0 | 0.000 | 0 | 0.000 | 0 |
a
Mature biofilms were allowed to form on silicone disks overnight. On the following day, disks were treated with EtOH at various concentrations for 4 h. Immediately following EtOH treatment, the disks were briefly sonicated and cultured in selective liquid medium for 24 h. Optical densities were recorded, and serial dilutions were plated onto selective medium to quantify microbial regrowth.