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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Aug 23;426(2):203–208. doi: 10.1016/j.bbrc.2012.08.063

Figure 1.

Figure 1

Elevated serotonin and SERT expression in mice exposed to hyperoxia (100% O2). A) Lung serotonin levels in mice exposed to room air and hyperoxic conditions. Lung homogenates were obtained from mice and subjected to capture ELISA to assess serotonin concentrations. B) Lung edema was measured using wet to dry ratio of mouse lungs exposed to hyperoxia and room air. C) Quantitative RT-PCR analysis to measure SERT mRNA expression in lung homogenates of mice exposed to hyperoxia and their room air controls. SERT mRNA was measured using reverse transcription and qPCR with SERT primers. 18S rRNA was used as internal control D) Relative SERT protein expression in mice lung homogenates was measured using Western blot analysis. **P<0.001 compared with room air controls.