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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Aug 23;426(2):203–208. doi: 10.1016/j.bbrc.2012.08.063

Figure 3.

Figure 3

Over expression of miR-16 downregulated SERT expression in A549 cells in vitro. A) Serotonin transporter expression varied dose dependently with the addition of different concentrations of serotonin. A549 cells were treated with increasing concentrations of serotonin and were subjected to qRT-PCR to assess SERT mRNA expression levels. SERT mRNA expression was normalized to GAPDH. B) qRT-PCR analysis of SERT in miR-16 overexpressed A549 cells post-treated with serotonin. A549 cells were transfected with miR-16; 4 h post-transfection, cells were treated with either 1 or 2 mM serotonin, and 24 h later, RNA was isolated and subjected to qRT-PCR analysis. All experiments were repeated at least 3 independent times. C) SERT protein expression was analyzed in miR-16 overexpressed A549 cells post-treated with serotonin. A549 cells were transfected with miR-16 or control plasmid and post-treated with serotonin for 24 h. Cell lysates were subjected to immunoblotting with the indicated antibodies. *P<0.01 and **P<0.001 compared with control.