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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Aug 23;426(2):203–208. doi: 10.1016/j.bbrc.2012.08.063

Figure 4.

Figure 4

miR-16 overexpression in A549 cells modulates ENaC and TGF-β expression via Nedd4 independent pathway. A) Immunoblot analysis of different subunits of ENaC in miR-16 and control plasmid transfected A549 cells. A549 cells transfected with miR-16 or control plasmid were post-treated with or without serotonin and protein lysates were analyzed for ENaC-α, -β and -γ by immunoblot. B) TGF-β concentration in cell supernatants was analyzed by capture ELISA. Human alveolar epithelial cells were transfected with miR-16 or control plasmid and 24 h post-transfection, the supernatants were subjected to ELISA. C) Mice were exposed to hyperoxia and room air, and lung homogenates were analyzed for Nedd4 levels by immunoblot. D) Nedd4 immunoblot in A549 cells transfected with miR-16 or control plasmid in the presence or absence of serotonin treatment post-transfection. *P<0.01 compared with control.