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. 2012 Nov 27;3:255. doi: 10.3389/fgene.2012.00255

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Copyright © 2012 Li, Cao and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Identification of NTPDase2-rtTA transgenic mice. (A) Map of NTPDase2-rtTA transgene construct. NTPDase2-rtTA transgenic mice were crossed with TeT-O-Cre and Rosa-LacZ transgenic mice to obtain triple-transgenic mice. After 10 days of Dox treatment of these triple-transgenic mice, expression of the transgene was analyzed by immunohistochemistry. (B) Immunohistochemical staining with anti-NTPDase2 revealed that NTPDase2 was expressed in lingual epithelia and papillae. (C) Immunohistochemical staining with anti-Cre revealed that Cre was expressed in lingual epithelia and papillae. (D) Immunohistochemical staining with anti-β-Gal detected LacZ in lingual epithelia and papillae. In situ hybridization with the NTPDase2 probe further revealed the expression of NTPDase2 in lingual epithelia and lingual papillae. (E) CV papillae, (F) fungiform papillae. B, basal cell layer; SB, suprabasal cell layer; CV, circumvallate papillae; FP, filiform papillae; FuP, fungiform papillae; ep, epithelia; TB, taste bud. Scale bar: (B–D), 12 μm; (E,F), 20 μm.