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Co-expression of GFP with GLAST in taste buds after 10 days of Dox induction. (A) Immunohistochemistry with anti-NTPDase2 showed the expression of NTPDase2 in taste buds and connective tissue around CV papillae. (B) After 10 days Dox induction, GFP is observed around the CV papillae. (C) GFP co-expression with GLAST in taste buds. (D) High magnification of taste bud from dotted frame in (C). CV, circumvallate papillae; TB, taste bud. Scale bar: (A–C), 50 μm; (D), 20 μm.
