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A) Schematic showing how mouse AV leaflets are harvested and sectioned. (B) Heart valve sections are positioned such that the cross-section of each leaflet is available for scanning, eliminating the concern of only observing surface phenomena with the AFM. (C) Slides are stained without fixation to ensure minimal disruption of the ECM. (D) Slides are subjected to scanning via PF-QNM mode of an AFM.
