Figure 1.

AKLIDES platform for automated evaluation of cell-based IIF testing and multiplex addressable microbead-based immunoassays analysis. (a) Schematic drawing of the main hardware components of the AKLIDES system based on a fluorescence microscope combined with different filtersets and objectives, a LED unit, a gray level camera, a movable scanning stage, and a controlling computer. (b) Flowchart of measurement and evaluation of automated HEp-2 IIF assay interpretation by the AKLIDES system including a sequential, multistage process of image acquisition, quality control, object segmentation, object description, and object classification [27]. (c) Application range of the AKLIDES system divided into evaluation of cell-based IIF testing for DSB and AAb detection as well as AAb analysis using bead-based multiplex assays.