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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: J Immunol. 2012 Nov 21;189(12):5549–5560. doi: 10.4049/jimmunol.1102221

Figure 2.

Figure 2

Direct presentation of Q489A and G490A determinants by B6-derived cells. B6-derived and tap1 knockout-derived cell lines expressing wt TAg or TAg variants expressing the Q489A or G490A substitutions were tested for their ability to stimulate IFN-γ production by either site I-specific CTL clone K-11 or bulk polyclonal site V-specific CTL. K-1,4,5 cells lack expression of the four known H-2b-restricted determinants. Cells were stained for intracellular IFN-γ production and analyzed by flow cytometry.