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Representative mRNA structures, showing the differences in secondary structure and ΔG around the ribosome binding site (RBS) for wild-type Usp45 signal peptide, Usp45SM2, and Usp45SM3. mRNAs from the transcript start site to 6 nucleotides after the end of the signal peptide sequence were entered into mfold to obtain the secondary structures (26). Structures with the lowest ΔG values are shown, with the RBS (AGGAGG) circled.
