Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| S. enterica serovar Typhimurium SL1344 | ||
| MB282 | Wild type; Strepr | 16 |
| MB427 | Rifr derivative of wild-type strain SL1344 | This study |
| SS371 | MB427 ΔpduD::kan; Strepr Kanr Rifr | This study |
| SS374 | MB427 ΔcobS::kan; Strepr Kanr Rifr | This study |
| SS377 | MB427 ΔeutK::kan; Strepr Kanr Rifr | This study |
| MB713 | SS371 with pMBpduD-X | This study |
| MB717 | SS377 with pMBeutRK | This study |
| MB784 | SL1344 ΔpduA-X::kan; Strepr Kanr | This study |
| MB789 | SL1344 ΔeutK-S::kan; Strepr Kanr | This study |
| MB792 | SL1344 ΔpduA-X::kan ΔeutK-S::cat; Strepr Kanr Cmr | This study |
| S. enterica serovar Typhimurium LT2 | ||
| TT22362 | Wild type | 17 |
| MB580 | Rifr derivative of wild-type strain TT22362 | This study |
| TT22344 | TT22362 pdu-12::MudA; Ampr | 17 |
| TT22341 | TT22362 ΔeutS-K; Tetr | 17 |
| TT20945 | TT22362 cbiA::MudJ ΔcbiB-cobT; Kanr | Gift of J. R. Roth |
| Dickeya dadantii | ||
| 3937 | Wild type | 18 |
| MB392 | Rifr derivative of strain 3937 | This study |
| Plasmids | ||
| pMBpduD-X | pBBR1-mcs5 with pduD-X from SL1344 cloned into the SacI site, driven by PlacZ; Gentr | This study |
| pMBeutRK | pBBR1-mcs5 with eutRK from SL1344 cloned into the SacI site, driven by PlacZ; Gentr | This study |
a
pduA-X consists of pduA, pduX, and all intergenic material; eutK-S is eutK, eutS, and all genetic material in between; and pduD-X contains pduD, pduX, and all intergenic material.