Table 2.
Cellular fatty acid compositions of strain TG408 and four reference strains
| Fatty acid | % of total fatty acidsa |
|||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | |
| C12:0 | 4.3 | 16.3 | 3.2 | 4.6 | 4.8 | |
| Iso-C12:0 | 2.8 | |||||
| C12:0 2-OH | 1.7 | 1.7 | 2.9 | |||
| C12:0 3-OH | 2.4 | 3.4 | 1.1 | |||
| C13:0 | 1.3 | |||||
| C13:0 3-OH | 1.3 | 4.3 | ||||
| C14:0 | 1.4 | 1.2 | 2.2 | 20.5 | 5.9 | 5.1 |
| Iso-C14:0 | 2.7 | |||||
| Iso-C14:0 3-OH | 2.8 | |||||
| C14:0 2-OH | 2.1 | |||||
| C14:0 3-OH | 6.6 | 2.8 | 7.1 | 9.2 | ||
| C16:0 | 38.2 | 30.1 | 18.9 | 42.2 | 12.3 | 11.8 |
| Iso-C16:0 | 10.5 | |||||
| C16:0 2-OH | 1.6 | |||||
| C16:0 3-OH | 1.3 | |||||
| C16:1 ω5c | 5.6 | 6.6 | ||||
| C16:1 ω7c | 31.3 | 3.8 | 16.4 | 16.1 | 3.9b | 3.7b |
| C16:1 ω11c | 1.4 | |||||
| C17:0 | 4.3 | |||||
| C17:0 cyclo | 2.4 | |||||
| C17:1 ω6c | 1.1 | 3.2 | ||||
| C17:1 ω8c | 1.5 | |||||
| C18:0 | 2.6 | 14.9 | 2.1 | 1.1 | ||
| C18:1 ω7c | 17.4 | 15.8 | 37.6 | 17.9 | 21.3 | 26.3 |
| C18:1 ω9c | 2.3 | |||||
| C19:0 cycloc | 15.3 | |||||
| C19:0 cyclo ω8c | 3.3 | 4.3 | ||||
Strains: 1, TG408; 2, Alkanibacter difficilis MN154.3T (36); 3, Hydrocarboniphaga effusa ATCC BAA332T (36); 4, Nevskia ramosa DSM 11499T (36); 5, Sinobacter flavus CW-KD4T (37); 6, Solimonas soli DCY12T (37). Lipid extraction of biomass, followed by methylation of the total extractable lipids, was used for strain 1, with whole-cell methylation protocols for strains 2 to 6. The latter also recovers lipopolysaccharide-derived OH fatty acids. Components that represented <1% in all strains are not included. The genus Sinobacter was recently reclassified as synonymous with Solimonas (37).
May also contain C16:1 ω6c, as reported in the original publications.
C19:0 cyclopropyl fatty acids; cyclo11-12 for strain 2 (36).