Table 2.

Primers and probe used in this study

Gene	Forward sequence (5′→3′)	Reverse sequence (5′-→3′)	Probe sequence (5′→3′)d	Amplicon size (bp)	Reference
gltA	GGGGACCAGCTCATGGTGG	AATGCAAAAAGAACAGTAAACA	NA	∼350	22
gltAa	GGGGACCAGCTCATGGTGG	CGTGGATCATAATTTTTATA	CCAAAACCCATAAGGCGGAAAGGATCATTT	∼143	19
gltAb	GTTATCCTATTGACCAA	CCAAAACCCATAAGGCG	NA	∼685	20
	AACTCTTGCCGCTATGG	TATTCTTCACAAGGAAC		∼401/387c
rpoB	GCACGATTYGCATCATCTTTTCC	CGCATTATGGTCGTATTTGTCC	NA	∼333	21
groEL	ATGGACAAAGTTGGCAATGAA	TTCCACCACCAGCAACAATA	NA	∼720	This study
ribC	TAACCGATATTGGTTGTGTTGAAG	TAAAGCTAGAAAGTCTGGCAACATAACG	NA	∼588	This study
ftsZ	CATATGGTTTTCATTACTGCYGGTATGG	TTCTTCGCGAATACGATTAGCAGCTTC	NA	∼515	21

^aqPCR.

^bSeminested PCR.

^cSeminested PCR product.

^dDually labeled oligonucleotide probe with 5′-6-carboxyfluorescein and 3′-black hole quencher 1. NA, not applicable.