Fig 1.

SDS-PAGE of partially purified thiosulfate dehydrogenase. (A) Lanes 1 to 5, samples from A. ferrooxidans ATCC 23270. (B) Lanes 7 to 9, samples from E. coli BL21(DE3) harboring pET-tsd. Lane 1, cell extract (20 μg); lane 2, soluble fraction (6.8 μg); lane 3, soluble fraction at pH 4 (11.35 μg); lane 4, CM-650M fraction (1.58 μg); lane 5, Butyl-650M fraction (1.04 μg); lane M, molecular markers; lane 6, cell extract from E. coli with pET21a (10 μg); lane 7, cell extract (9.9 μg); lane 8, soluble protein at pH 4.0 (9.9 μg); lane 9, Butyl-650M fraction (1.53 μg).