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Effect of thiosulfate concentration on TSD activity in the Butyl-650M fraction of A. ferrooxidans ATCC 23270 (●) or E. coli BL21(DE3) harboring pET-tsd (○). Experiments were carried out at pH 2.5 and 40°C in a mixture containing 1 mM ferricyanide and 50 mM Na-sulfate.
