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Stoichiometry of thiosulfate consumption and tetrathionate formation by TSD from A. ferrooxidans. Experiments were carried out at pH 2.5 and 20°C in a mixture containing 5 mM Na-thiosulfate, 1 mM ferricyanide, and 6.88 μg · ml−1 of TSD (Butyl-650M fraction) from A. ferrooxidans. Symbols: circles, thiosulfate concentrations in the presence (●) or absence (○) of the enzyme; triangles, tetrathionate concentrations in the presence (▲) or absence (△) of the enzyme.
