Table 1.
Purification of thiosulfate dehydrogenase at the optimal pH of 2.5 from the soluble fraction of tetrathionate-grown A. ferrooxidans ATCC 23270a
| Purification step | Total protein (mg) | Sp act (μmol min−1 mg−1) | Total activity (μmol min−1) | Recovery (%) | Purification (fold) |
|---|---|---|---|---|---|
| Cell extract | 200.8 | 0.05 | 10.0 | 100 | 1 |
| Soluble fraction | 55.1 | 0.09 | 4.9 | 49 | 1.8 |
| Soluble fraction at pH 4 | 11.1 | 0.20 | 2.2 | 22 | 4.0 |
| CM-650M fraction | 1.3 | 2.28 | 3.0 | 30 | 45.6 |
| Butyl-650M fraction | 0.1 | 3.16 | 0.3 | 3 | 63.2 |
a
Enzyme activity was measured at pH 2.5 and 40°C in a mixture containing 1 mM ferricyanide and 10 mM thiosulfate.