Fig 1.
Thin-layer chromatography (TLC) analysis of crude biosurfactant extract from the cultures sampled at day 15. Panels A, B, and C show the presence and relative amounts of glycolipids, phospholipids, and lipopeptides from the n-hexadecane (C16), n-tetracosane (C24), and n-hexatriacontane (C36) cultures, respectively. In panel A, 2 glycolipids (16-A, Rf, 0.59; 16-B, Rf, 0.51) were detected from the C16 culture, whereas glycolipids 24-C (Rf, 0.60) and 36-D (Rf, 0.59) were detected from the C24 and C36 cultures, respectively. In panel B, phospholipids (Rf, 0.62) were detected only in the C24 culture. In panel C, lipopeptides were detectable only in the C36 culture, with 2 compounds detected: CL-a (Rf, 0.79) and CL-b (Rf, 0.56).